The MM-100 Metabolic Monitor System measures oxygen consumption (VO2) and CO2 production (VCO2) of small animals using the open circuit technique. The animal is placed in a closed chamber with a known flow of fresh air passing through it. Oxygen and carbon dioxide concentrations are measured at the inlet and outlet of the chamber. The three measurements (flow, O2, and CO2) enable the computation of oxygen consumption, CO2 production, and Respiratory Exchange Ratio (RER), or VCO2/VO2.
Oxygen analyzer type: paramagnetic
Linearity (0 - 100%): +0.1% O2
Repeatability: < 0.01% O2/hour
Zero stability: +0.002% O2/hour
Response time: 2.0 seconds
Operating temperature: 0 - 65oC
Maximum pressure: 15 psi
Maximum sample flow: 250 ml/min
Instrument measurement range: 0 - 24.5%
O2 Carbon Dioxide analyzer type: single-beam infrared
Operating range: 0 - 5000ppm (0.5%)
CO2 Linearity (full scale): 1%
FS Zero drift: 2%
FS/week Sensor cell temperature: controlled at 60oC
Warm-up time: <15 min
Maximum sample flow: 200 ml/min
Instrument measurement range: 0 - 0.41%CO2
Flow transducer: thermal mass flowmeter
Flow range: 0 - 10,000 ml/min
Linear flow range: 50 - 10,000 ml/min
Response time: < 2S
Operating temperature: -20 - 70oC
Main unit analog outputs: flow, O2, CO2
Analog signal outputs (rear panel): BNC jacks
Flow output: 1mV/ml/min
Flow output zero offset: 1.250V
O2 output: 0.1667V/%O2
CO2 output: 10V/%CO2
Serial data output format: 9600 baud, 8 data bits, no parity, 1 stop bit
Serial data interval: complete set of readings sent 1 per minute
Serial output connector (rear panel): DB9 female
Dimensions, main unit: 19W x 5.25H x 15D in.
Power requirements: 120/240V (switchable), 200VA
MMX-2, MMX-8, MMX-16 MMX-Series Expanders allow the user to simultaneously monitor 2, 8, or 16 animals with the MM-100 Metabolic Monitoring System.
Simple set up and operation
The animal is placed in the provided chamber and allowed to equilibrate for 5 - 10 minutes. All gas and flow measurements, and computed results are shown on the front panel LCD display panel. A serial port connection sends the measurements to any attached computer. Using the included WindowsTM software, the readings for O2 consumption and CO2 production, along with their moving time averages, are plotted. Data can be logged to simple ASCII files for further analysis. Standardization against room air is automatically performed at intervals, so the accuracy of the system is maintained for long intervals of monitoring.
Front panel display panel shows all gas, flow, and computed measurements, as well as elapsed time and chamber being monitored.
Multiple chamber systems
Expansion to multiple animals is straightforward. Using the MMX-4 or MMX-16 (for four and sixteen animals, respectively) expansion units, gas sampling and measurement is automatically sequenced between the chambers. This results in an accurate, economical system for screening large numbers of animals for these important physiological parameters.
MM-COMM Monitoring Software
Each MM-100 system includes WindowsTM software for graphically displaying and logging the metabolic data. This software provides a Data Panel showing all the current raw and computed measurements. Data is logged in a simple ASCII format for directly importing it into a spreadsheet or other analysis program. Each record is tagged with the animal number and time of the measurement.
The MM-100 comes complete with all necessary components for a single-chamber measurement. This includes the main MM-100 unit, small animal chamber, air pump, drying tubes, and required tubing. The only consumable item is the Drierite material for drying the gas sample.