This video describes how to calibrate the microCapStar end tidal CO2 analyzer.
- Fast Response
- Accurate and stable monitoring
- Ultra-low sample flow rate
- Linear output voltage
- End-tidal peak or continuous readings
- Measures respiratory rate
- For mice, rats, and larger animals
- Carbon dioxide measurement: single beam, non-dispersive infrared
- Measurement range: 0 - 9.9% (0 - 76.0mmHg) CO2
- Accuracy: 0.15% (1.1mmHg)
- Resolution (internal and analog output): 0.01% (0.1mmHg)
- Resolution (display): 0.1% (1.0mmHg)
- Linearity: 0.1% CO2
- Repeatability: 0.1% CO2
- Response time (T10 - T90): 75mS at 70 ml/min through cell
- Sample cell materials: sapphire and stainless steel
- Analog output scaling: 1.1V/% (0-10V)
- Response time (T10 - T90): 150mS at 50 ml/min sampling
- Zero stability: 0.2% (8 hours), 0.3% (24 hours)
- Interference effects: 50% N2O: 0.1% at 0% CO2, 0.6% at 5% CO2 (uncomp.)
- Maximum sample cell pressure: +5 psig
- Interference effects: vaporized anaesthetic agents: negligible
- Operating temperature range: 5-40° C
- Optical bench temperature: 48° C, controlled
- Warm-up time: 4 min to 0.2%, 10 min to 0.1%
- ETCO2 trend display: 5 minute graphical scrolling display
- Sample flow (sample inlet - carrier flow out): 10ml/min nominal, adjustable from 5.0 - 20ml/min
- Sample tubing connections (carrier flow, sample inlet): Luer female
- Calibration controls: Zero, Span
- ETCO2 alarm adjustment range: 1.0 - 9.9% (8 - 75mmHg)
- Respiratory rate measurement range: 5 - 200 breaths/min
- Signal outputs (rear panel): BNC jacks
- CO2 output scaling: 1.0V / %CO2
- Respiratory rate output scaling: 0.05V / bpm
- Alarm output: 5V if alarm condition, 0V if no alarm
- Serial data output format: 2400 baud, 8 data, no parity, 1 stop bit
- Electrical requirements: 120VAC/220VAC switchable, 35VA
- Dimensions: 19W x 5.25H x 16D in., 49W x 13H x 41D cm
- Weight: 10 lbs. (4.5kg)
The microCapStar End-Tidal Carbon Dioxide Analyzer provides accurate end-tidal or continuous measurement of expired CO2 in animals as small as mice. It features very low sample flow requirements, rapid response time, and long-term stability. Respiratory rate (RR) is computed using the excursions of the CO2 waveform. The CO2 and RR measurements, as well as a trend plot of the end-tidal values, are displayed on the graphics LCD screen.
The heart of the microCapStar is a temperature-controlled infrared CO2 sensor. Low sample flow and rapid response is achieved with a carrier gas system employing digitally-controlled active flow management. This technique automatically maintains the ratio of carrier flow to sample flow, which is essential for accurate measurements. The heated measurement cell prevents water condensation, even during long-term measurement sessions. The standard sample tubing set is 1m long, but lengths over 2.5m are possible.
The front-panel display shows CO2 concentration (either instantaneous or ETCO2) in either percent or mmHg, and a 5-minute trend plot of ETCO2.. Calibration is performed with a single calibration gas and room air. An adjustable ETCO2 alarm provides a warning when end-tidal values fall out of a user-adjustable preset range. All adjustments are performed using a single knob. Built-in diagnostics monitoring warn of plugged sample tubing or other fault conditions.
The advanced features, reliability, and ease of operation of the microCapStar make it the perfect companion to our Small Animal Ventilators for monitoring respiratory status. With the AVS-1 Advanced Ventilator System, it can be used to control end-tidal CO2. An accessory pack containing spare low-volume sample tubing and a variety of connectors and fittings is included with the instrument. A range of accessories is available to ensure easy setup and convenient operation.
CO2 monitoring is widely recognized as an important measure of the respiratory status of experimental animals. It is useful in setting ventilator parameters, and serves to gauge depth of anesthesia in unassisted, spontaneously breathing animals as well. The microCapStar extends this important technique to the realm of small experimental animals.